Aurora B and DmINCENP fail to localize at the kinetochores of aust spermatocytes. (A–C) Cells fixed and stained for DNA (blue), α-tubulin (green), and either CID (A), DmINCENP (B), or aurora B (C). In wild-type cells, all localize to kinetochores. In aust mutants, CID is present on kinetochores, whereas DmINCENP and aurora B are absent. Bar, 10 μm.