Alignment of the currently known procaspase-14 amino acid sequences. Sequence analysis indicates that a hydrophobic patch in the protease-sensitive loop is conserved. Only part of the alignment is shown here, including the C-terminal part of the p20 subunit, the protease-sensitive loop, and the N-terminal part of the p10 subunit. The darker the yellow, the more the amino acids are conserved between species. The catalytic QACRG box is delineated with a green box. The conserved hydrophobic patch is delineated with a red box, and the cleavage site in human caspase-14 is indicated with a red arrow. The alignment was performed using ClustalW (Mega version 3.1; Kumar et al., 2004) and was manually optimized in the protease-sensitive loop region. JalView 2.3 (Clamp et al., 2004) was used for visualization.