Figure 6. Sustained activation of STAT5 is required for histone acetylation in the β-casein promoter. (A and B) ChIP assays measuring the acetylated histone levels in the β-casein promoter. (A) EpH4 cells on polyHEMA were treated with Prl or Prl plus 2% lrECM for different times before the analysis. (B) EpH4 cells were treated with AG490 to block the sustained STAT5 activation as shown in Fig. 4 A before the ChIP analysis. The PCR results were quantified by AlphaEaseFC software, and the values of ChIP DNA were normalized to input DNA. Fold enrichments were determined by dividing the normalized values from treated cells by that of untreated cells.
Figure 6.

Sustained activation of STAT5 is required for histone acetylation in the β-casein promoter. (A and B) ChIP assays measuring the acetylated histone levels in the β-casein promoter. (A) EpH4 cells on polyHEMA were treated with Prl or Prl plus 2% lrECM for different times before the analysis. (B) EpH4 cells were treated with AG490 to block the sustained STAT5 activation as shown in Fig. 4 A before the ChIP analysis. The PCR results were quantified by AlphaEaseFC software, and the values of ChIP DNA were normalized to input DNA. Fold enrichments were determined by dividing the normalized values from treated cells by that of untreated cells.

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