Figure 4. Laminin-111 regulates sustained STAT5 activation and casein transcription. (A) Immunofluorescence analysis of PrlR and ZO-1 in EpH4 cells on polyHEMA that were treated with Prl alone (pHEMA) or Prl plus 2% lrECM (3D lrECM) for 24 h. Bar, 25 μm. (B) Western blot analysis of phosphorylated STAT5 in EpH4 cells. The cells on polyHEMA were treated with Prl plus collagen or laminin-111 at 120 μg/ml for 24 h. (C) Quantification of β-casein mRNA levels by real-time RT-PCR. EpH4 cells on polyHEMA were treated with Prl plus collagen or laminin-111 at 120 μg/ml for 24 h. The bar graph represents the mean ± SEM. **, P < 0.01; n = 4.
Figure 4.

Laminin-111 regulates sustained STAT5 activation and casein transcription. (A) Immunofluorescence analysis of PrlR and ZO-1 in EpH4 cells on polyHEMA that were treated with Prl alone (pHEMA) or Prl plus 2% lrECM (3D lrECM) for 24 h. Bar, 25 μm. (B) Western blot analysis of phosphorylated STAT5 in EpH4 cells. The cells on polyHEMA were treated with Prl plus collagen or laminin-111 at 120 μg/ml for 24 h. (C) Quantification of β-casein mRNA levels by real-time RT-PCR. EpH4 cells on polyHEMA were treated with Prl plus collagen or laminin-111 at 120 μg/ml for 24 h. The bar graph represents the mean ± SEM. **, P < 0.01; n = 4.

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