β-Casein transcription corresponds with lrECM-induced sustained STAT5 activation. (A) EpH4 cells on polyHEMA were treated with Prl alone or Prl plus 2% lrECM for different intervals. STAT5 phosphorylation was detected by Western blotting. The Western blot results were quantified by AlphaEaseFC software and expressed as relative levels of phosphorylated STAT5 to total STAT5 (n = 3). (B) Quantification of β-casein mRNA levels by real-time RT-PCR. EpH4 cells on polyHEMA were treated with Prl alone or Prl plus 2% lrECM for different time points before RNA extraction. (C) Western blot analysis of nuclear levels of STAT5 in EpH4 cells. Cells on polyHEMA were treated with Prl alone or Prl plus 2% lrECM for 30 min or 24 or 48 h.