Figure 2.
Figure 2. Cbx1−/− neonates do not inflate their lungs, and E19 Cbx1−/− embryos exhibit reduced clusters of postsynaptic AChRs within diaphragm muscle. (A) Hematoxylin and eosin–stained transverse sections through the midbody of wild-type (left) and Cbx1−/− (right) neonates showing lack of inflation of the lungs in the Cbx1−/− neonates. Al, alveolus; Br, bronchiole; IM, intercostal muscle; Lu, lung; SC, spinal cord. Bars: (top) 450 μm; (middle) 150 μm; (bottom) 50 μm. (B) Staining of E19 wild-type diaphragms (top) with antibodies to NF (red) shows that the diaphragm is clearly innervated. Bungarotoxin-positive (BGX; green) AChRs are clustered around the nerve and its branches. The clusters of bungarotoxin-positive AChRs are much reduced in E19 Cbx1−/− diaphragms (middle and bottom), and, in some cases, the branching of the innervating nerve is also reduced (bottom; see red NF staining). Bar, 25 μm.

Cbx1−/− neonates do not inflate their lungs, and E19 Cbx1−/− embryos exhibit reduced clusters of postsynaptic AChRs within diaphragm muscle. (A) Hematoxylin and eosin–stained transverse sections through the midbody of wild-type (left) and Cbx1−/− (right) neonates showing lack of inflation of the lungs in the Cbx1−/− neonates. Al, alveolus; Br, bronchiole; IM, intercostal muscle; Lu, lung; SC, spinal cord. Bars: (top) 450 μm; (middle) 150 μm; (bottom) 50 μm. (B) Staining of E19 wild-type diaphragms (top) with antibodies to NF (red) shows that the diaphragm is clearly innervated. Bungarotoxin-positive (BGX; green) AChRs are clustered around the nerve and its branches. The clusters of bungarotoxin-positive AChRs are much reduced in E19 Cbx1−/− diaphragms (middle and bottom), and, in some cases, the branching of the innervating nerve is also reduced (bottom; see red NF staining). Bar, 25 μm.

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