Figure 5.
![Figure 5. L-type channel activity produces phosphoCaMKII puncta near the cell surface. (A) SCG neurons stimulated with 40 mM K+ for 0, 10, or 60 s and immediately fixed and stained for Map2 (red) and pCaMKII (green). Arrows point to pCaMKII puncta (magnified in the inset). Background pCaMKII staining was subtracted to highlight punctate staining (see Materials and methods). Bar, 20 μm (B) pCaMKII puncta weight from experiments as in A. (C) Nimodipine blocks pCaMKII puncta formation. (D) pCaMKII puncta weight from cells treated for 60 s as shown on a log–log plot against the rise in bulk Ca2+ (Δ[Ca2+]i are taken from Fig. 4). For all, n = 3 independent experiments. Error bars represent SEM.](https://cdn.rupress.org/rup/content_public/journal/jcb/183/5/10.1083_jcb.200805048/5/jcb_200805048r_rgb_fig5.jpeg?Expires=1773585510&Signature=cttUteLmyYocJqTR8M9fUaqwm0sMn4iSRie~VcBBenmcXVpZKaRUzLfvEHHjU4zbs1zeRPciyDBQl3SMC~U9l3HYEicAVJ5AZVJVHH6rzYFElqNDCKc7xB96O-ICadSu-b55zFONytYHIvFDqHKrpOHE5ebwZStPu5xRdCh5mS5MRVKvc7xoxDKROC8oJX9TEJicJ0Gp-ANCCk1HiKwHMWkaMo6eVCmAMPxL4XL4i3GPtgzRGJpMZf98QaogcLoFnKyLLauqW0aSfg2pBw-NHUHdwAHSj00QAOCTbu~E~YnFyY~dHbyqJySqZ991iAGaKkJu~ec82izXQWYe0LHktA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
L-type channel activity produces phosphoCaMKII puncta near the cell surface. (A) SCG neurons stimulated with 40 mM K+ for 0, 10, or 60 s and immediately fixed and stained for Map2 (red) and pCaMKII (green). Arrows point to pCaMKII puncta (magnified in the inset). Background pCaMKII staining was subtracted to highlight punctate staining (see Materials and methods). Bar, 20 μm (B) pCaMKII puncta weight from experiments as in A. (C) Nimodipine blocks pCaMKII puncta formation. (D) pCaMKII puncta weight from cells treated for 60 s as shown on a log–log plot against the rise in bulk Ca2+ (Δ[Ca2+]i are taken from Fig. 4). For all, n = 3 independent experiments. Error bars represent SEM.
