CycB localizes to the kinetochores before NEB. RLC-GFP (A, cyan), GFP-Polo (B, cyan), and GFP-Cid (C, cyan) embryos were injected with 13 μM CycB-R (red) at interphase of cycle 12 (A) or 13 (B and C). Arrows highlight the punctate nuclear localization of CycB-R (A) and its colocalization with GFP-Polo (B) and GFP-Cid (C) before NEB. Time is given in minutes/seconds. Bars, 5 μm.