Figure 4. CycB injections overcome the S-phase checkpoint–induced cytoplasmic cell cycle arrest, and to a lesser extent, nuclear cell cycle arrest. (A–C) Wild-type (WT, A–B″) or grp mutant embryos (C) expressing RLC-GFP were injected with CHX and Aph together (CHX + Aph) at metaphase of cycle 12. The embryos were injected with CycB at the onset of interphase (A, early CycB injection) or 10 min after the onset of interphase (B–C, late CycB injection). The schematic describes the injection and imaging sequence. Arrowheads mark the sites of injection. Blue outlines mark areas with cytoplasmic CDK1 activity, and orange outlines mark areas where NEB occurs. Time is given in minutes/seconds. Bars, 20 μm. (D) Timing of cytoplasmic and nuclear CDK1 activation was measured as described in Fig. 2. Data are represented as mean ± AVD. n, number of embryos injected.
Figure 4.

CycB injections overcome the S-phase checkpoint–induced cytoplasmic cell cycle arrest, and to a lesser extent, nuclear cell cycle arrest. (A–C) Wild-type (WT, A–B″) or grp mutant embryos (C) expressing RLC-GFP were injected with CHX and Aph together (CHX + Aph) at metaphase of cycle 12. The embryos were injected with CycB at the onset of interphase (A, early CycB injection) or 10 min after the onset of interphase (B–C, late CycB injection). The schematic describes the injection and imaging sequence. Arrowheads mark the sites of injection. Blue outlines mark areas with cytoplasmic CDK1 activity, and orange outlines mark areas where NEB occurs. Time is given in minutes/seconds. Bars, 20 μm. (D) Timing of cytoplasmic and nuclear CDK1 activation was measured as described in Fig. 2. Data are represented as mean ± AVD. n, number of embryos injected.

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