Figure 5. In vivo, Ski and ser780 pRb... | Rockefeller University Press

Figure 5.

$Figure 5. In vivo, Ski and ser780pRb are found in the cytoplasm of developing Schwann cells, where they interact, whereas ser780pRb is mostly restricted to the nucleus of myelinating Schwann cells. (A and B) Western blot analysis of Ski (A) and Rb and ser780pRb (pRb; B) expression in developing rat sciatic nerves, and graphs representing the densitometry of the bands normalized to the loading control β-actin or GAPDH. Statistical analyses were performed using two-tailed t tests on three groups of animals for each age. (C and D) Images of single confocal sections of Ski (red; C) or ser780pRb (pRb; green; D) and DAPI (blue) immunostainings on longitudinal sections of P0 and adult rat sciatic nerves. Arrows point out Schwann cell nuclei. (E) Images of single confocal sections of Ski (red) and ser780pRb (pRb; green) coimmunostainings and DAPI (blue) labeling on teased nerve fibers of P0 and adult rat sciatic nerves. In C–E, Ski appears pink, and pRb appears turquoise when nuclear. In E, the overlay of Ski and pRb appears yellow. Arrows indicate examples of the colocalization of Ski and ser780pRb. Insets are magnifications of the regions outlined by boxes. (F) Immunoprecipitation of Ski and Western blot of ser780pRb (pRb) or Ski in the cytoplasmic (C) and nuclear (N) fractions of P7 and adult rat sciatic nerve lysates. Error bars represent SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001.$

In vivo, Ski and ^ser780pRb are found in the cytoplasm of developing Schwann cells, where they interact, whereas ^ser780pRb is mostly restricted to the nucleus of myelinating Schwann cells. (A and B) Western blot analysis of Ski (A) and Rb and ^ser780pRb (pRb; B) expression in developing rat sciatic nerves, and graphs representing the densitometry of the bands normalized to the loading control β-actin or GAPDH. Statistical analyses were performed using two-tailed t tests on three groups of animals for each age. (C and D) Images of single confocal sections of Ski (red; C) or ^ser780pRb (pRb; green; D) and DAPI (blue) immunostainings on longitudinal sections of P0 and adult rat sciatic nerves. Arrows point out Schwann cell nuclei. (E) Images of single confocal sections of Ski (red) and ^ser780pRb (pRb; green) coimmunostainings and DAPI (blue) labeling on teased nerve fibers of P0 and adult rat sciatic nerves. In C–E, Ski appears pink, and pRb appears turquoise when nuclear. In E, the overlay of Ski and pRb appears yellow. Arrows indicate examples of the colocalization of Ski and ^ser780pRb. Insets are magnifications of the regions outlined by boxes. (F) Immunoprecipitation of Ski and Western blot of ^ser780pRb (pRb) or Ski in the cytoplasmic (C) and nuclear (N) fractions of P7 and adult rat sciatic nerve lysates. Error bars represent SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

This Feature Is Available To Subscribers Only