Figure 4. TGFβ promotes Ski and Rb... | Rockefeller University Press

Figure 4.

$Figure 4. TGFβ promotes Ski and Rb localization as a complex in the cytoplasm of Schwann cells but not of epithelial cells. (A–C) Images of single confocal sections of the coimmunostaining of Ski (red) and Rb (green; A) or Ski (red) and ser780pRb (pRb; green; B) in rat Schwann cells or in WB-F344 cells (C) cultured in DM or treated with TGFβ (the overlay appears yellow). Arrows indicate examples of the colocalization of Ski and Rb (A) and Ski and ser780pRb (B). Insets are magnifications of the regions outlined by boxes. (D) Immunoprecipitation of Ski and Western blotting of Rb or Ski in the cytoplasmic (C) and nuclear (N) fractions of rat Schwann cells treated with TGFβ for 36 h, and Western blot of lamin (nuclear marker) and GAPDH (cytoplasmic marker) performed on lysates used for immunoprecipitation (input). The arrow indicates a nonspecific band or a degradation product.$

TGFβ promotes Ski and Rb localization as a complex in the cytoplasm of Schwann cells but not of epithelial cells. (A–C) Images of single confocal sections of the coimmunostaining of Ski (red) and Rb (green; A) or Ski (red) and ^ser780pRb (pRb; green; B) in rat Schwann cells or in WB-F344 cells (C) cultured in DM or treated with TGFβ (the overlay appears yellow). Arrows indicate examples of the colocalization of Ski and Rb (A) and Ski and ^ser780pRb (B). Insets are magnifications of the regions outlined by boxes. (D) Immunoprecipitation of Ski and Western blotting of Rb or Ski in the cytoplasmic (C) and nuclear (N) fractions of rat Schwann cells treated with TGFβ for 36 h, and Western blot of lamin (nuclear marker) and GAPDH (cytoplasmic marker) performed on lysates used for immunoprecipitation (input). The arrow indicates a nonspecific band or a degradation product.

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