Figure 2.
Figure 2. TGFβ down-regulates Ski in epithelial cells but not in Schwann cells and mediates Rb up-regulation in Schwann cells but not in epithelial cells. Western blot analysis of Ski (A), Rb (C), and ser780pRb (pRb; E) in Schwann cells cultured in DM alone or treated with TGFβ or dbcAMP. Western blot analysis of Ski (B), Rb (D), and ser780pRb (pRb; F) in WB-F344 cells cultured in DM or treated with TGFβ. GAPDH or β-actin were used as loading controls. Graphs represent the densitometry analysis of the protein of interest normalized to the loading control. Statistical analyses were performed using two-tailed t tests on at least three independent experiments. Error bars represent SEM. *, P < 0.05; ***, P < 0.001. A and B, n = 4; C, n ≥ 3; D–F, n = 3.

TGFβ down-regulates Ski in epithelial cells but not in Schwann cells and mediates Rb up-regulation in Schwann cells but not in epithelial cells. Western blot analysis of Ski (A), Rb (C), and ser780pRb (pRb; E) in Schwann cells cultured in DM alone or treated with TGFβ or dbcAMP. Western blot analysis of Ski (B), Rb (D), and ser780pRb (pRb; F) in WB-F344 cells cultured in DM or treated with TGFβ. GAPDH or β-actin were used as loading controls. Graphs represent the densitometry analysis of the protein of interest normalized to the loading control. Statistical analyses were performed using two-tailed t tests on at least three independent experiments. Error bars represent SEM. *, P < 0.05; ***, P < 0.001. A and B, n = 4; C, n ≥ 3; D–F, n = 3.

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