Figure 2. Association of dynein accessory proteins with prometaphase kinetochores in dynein tail–expressing cells. COS7 cells transfected with the myc-C1140 construct were treated with nocodazole for 3 h. The presence of dynein accessory polypeptides was tested by immunofluorescence microscopy (A–C) or by use of GFP-tagged coexpressed polypeptides (D and E). Kinetochore staining of the respective proteins in nocodazole-treated control cells are shown in A′–E′. Bars, 5 μM.
Figure 2.

Association of dynein accessory proteins with prometaphase kinetochores in dynein tail–expressing cells. COS7 cells transfected with the myc-C1140 construct were treated with nocodazole for 3 h. The presence of dynein accessory polypeptides was tested by immunofluorescence microscopy (A–C) or by use of GFP-tagged coexpressed polypeptides (D and E). Kinetochore staining of the respective proteins in nocodazole-treated control cells are shown in A′–E′. Bars, 5 μM.

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