Figure 1. Displacement of endogenous dynein HC from kinetochores by dynein tail fragments. (A) Dynein fragments used in this study. (B) Mitotic index produced by expression of tail fragments in COS7 cells (n = 500). (C) Localization of the tail fragment myc-C1140 to prometaphase kinetochores in COS7 cells. (D and E) Displacement of endogenous dynein HC from prometaphase kinetochores in COS7 cells expressing the myc-C1140 tail fragment. (D) Kinetochore staining with an antibody recognizing endogenous dynein HC motor domain is highly reduced in cells expressing the tail. (E) Quantitation of dynein HC immunofluorescence at nonaligned prometaphase kinetochores (n = 35). P = 0.0025; two-tailed t test. (F) Kinetochore staining by antibody to endogenous dynein HC in control cells. Error bars indicate SD from mean from three independent experiments. Bar, 5 μM.
Figure 1.

Displacement of endogenous dynein HC from kinetochores by dynein tail fragments. (A) Dynein fragments used in this study. (B) Mitotic index produced by expression of tail fragments in COS7 cells (n = 500). (C) Localization of the tail fragment myc-C1140 to prometaphase kinetochores in COS7 cells. (D and E) Displacement of endogenous dynein HC from prometaphase kinetochores in COS7 cells expressing the myc-C1140 tail fragment. (D) Kinetochore staining with an antibody recognizing endogenous dynein HC motor domain is highly reduced in cells expressing the tail. (E) Quantitation of dynein HC immunofluorescence at nonaligned prometaphase kinetochores (n = 35). P = 0.0025; two-tailed t test. (F) Kinetochore staining by antibody to endogenous dynein HC in control cells. Error bars indicate SD from mean from three independent experiments. Bar, 5 μM.

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