Knockdown of dLKR/SDH results in global up-regulation of H3R17me2. Drosophila l(2)mbn cells were treated with double-stranded RNA to Ndfip2 (control) or dLKR/SDH (dLKR/SDH) and treated with either ethanol (−) or ecdysone (+) for 4 h. Lysates were prepared, and 75 μg was separated by SDS-PAGE, transferred to a membrane, and blotted with histone H3 methyl–specific antibodies as shown. The same membranes were blotted with HP1-β antibody as a loading control. For the detection of H3R17me2, 100 μg of protein was loaded onto the gel.