Figure 6. dLKR/SDH regulates the dynamics of histone H3R17me2. (A) 107 l(2)mbn cells were treated with either double-stranded RNA to control (Ndfip2) or dLKR/SDH for 48 h. Cells were then treated with ethanol (0) or ecdysone. ChIP assays were performed as outlined in Fig. 5. RNA was purified from the first extraction step and converted to cDNA, and the levels of dLKR/SDH transcript were detected by RT-PCR (dLKR/SDH transcript). (B) Antibody-enriched genomic DNA used in A and input genomic DNA per treatment were used in semiquantitative real-time PCR. Relative levels were determined by comparing the levels of DNA from the immunoprecipitated samples to the corresponding input genomic DNA. Error bars represent SEM.
Figure 6.

dLKR/SDH regulates the dynamics of histone H3R17me2. (A) 107 l(2)mbn cells were treated with either double-stranded RNA to control (Ndfip2) or dLKR/SDH for 48 h. Cells were then treated with ethanol (0) or ecdysone. ChIP assays were performed as outlined in Fig. 5. RNA was purified from the first extraction step and converted to cDNA, and the levels of dLKR/SDH transcript were detected by RT-PCR (dLKR/SDH transcript). (B) Antibody-enriched genomic DNA used in A and input genomic DNA per treatment were used in semiquantitative real-time PCR. Relative levels were determined by comparing the levels of DNA from the immunoprecipitated samples to the corresponding input genomic DNA. Error bars represent SEM.

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