Figure 4. The RE is required for furrow maintenance. (A) GFP-Moesin (green) and Rhodamine-tubulin (red) in WT embryos. From cycle-13 interphase to metaphase, three z sections are shown from 1 to 3 μm below the cortex. (B) Same experiment as in A in nuf embryos. F-actin was progressively lost at the furrow (arrows) as furrows invaginated, and this loss started at the sections closer to the invaginating tips (arrowheads; compare 6:30 to 0:00 at different depths). Spindle fusions (asterisks) occurred where furrows were broken. See Video 3 (available). (C) Time-lapse images of Rab11 cycle-13 furrow progression. Rhodamine-labeled actin was gradually lost at the furrow (arrows). (D) GFP-Dlg (green) and Rhodamine-actin (red) in nuf embryos. Yellow arrowheads indicate loss of membrane at the furrow over time. Loss of F-actin at the furrow generally precedes loss of membrane (arrows). See Video 4 (available at http://www.jcb.org/cgi/content/full/jcb.200712036/DC1). Bars, 10 μm.
Figure 4.

The RE is required for furrow maintenance. (A) GFP-Moesin (green) and Rhodamine-tubulin (red) in WT embryos. From cycle-13 interphase to metaphase, three z sections are shown from 1 to 3 μm below the cortex. (B) Same experiment as in A in nuf embryos. F-actin was progressively lost at the furrow (arrows) as furrows invaginated, and this loss started at the sections closer to the invaginating tips (arrowheads; compare 6:30 to 0:00 at different depths). Spindle fusions (asterisks) occurred where furrows were broken. See Video 3 . (C) Time-lapse images of Rab11 cycle-13 furrow progression. Rhodamine-labeled actin was gradually lost at the furrow (arrows). (D) GFP-Dlg (green) and Rhodamine-actin (red) in nuf embryos. Yellow arrowheads indicate loss of membrane at the furrow over time. Loss of F-actin at the furrow generally precedes loss of membrane (arrows). See Video 4 . Bars, 10 μm.

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