Figure 1. F-actin is rapidly turned over in the furrow through depolymerization and repolymerization. The schematic illustrates the focal plane relative to the furrow. Blue, nuclei; green, membrane. (A) Rhodamine-actin (red) was rapidly incorporated into preexisting (arrows) and newly forming (arrowheads) furrows marked by GFP-Moesin (green) at cycle-12 prophase. (B) GFP-Moesin and Rhodamine-actin showed a dramatic signal decrease at the furrow within 4 min after LatA but not DMSO injection. (C) Areas measured to calculate net furrow intensity (furrow intensity – nuclear intensity). (D and E) FRAP analysis of actin turnover during cycle-13 prophase. (D) White box indicates photobleached area. Yellow boxes indicate an example of a furrow region used for quantifications in E. See Video 1 (available). (E) Relative fluorescence intensities of GFP-Moesin and Rhodamine-actin at the furrows (y axis) after photobleaching (from D; n = 10 embryos). Bars, 10 μm.
Figure 1.

F-actin is rapidly turned over in the furrow through depolymerization and repolymerization. The schematic illustrates the focal plane relative to the furrow. Blue, nuclei; green, membrane. (A) Rhodamine-actin (red) was rapidly incorporated into preexisting (arrows) and newly forming (arrowheads) furrows marked by GFP-Moesin (green) at cycle-12 prophase. (B) GFP-Moesin and Rhodamine-actin showed a dramatic signal decrease at the furrow within 4 min after LatA but not DMSO injection. (C) Areas measured to calculate net furrow intensity (furrow intensity – nuclear intensity). (D and E) FRAP analysis of actin turnover during cycle-13 prophase. (D) White box indicates photobleached area. Yellow boxes indicate an example of a furrow region used for quantifications in E. See Video 1 . (E) Relative fluorescence intensities of GFP-Moesin and Rhodamine-actin at the furrows (y axis) after photobleaching (from D; n = 10 embryos). Bars, 10 μm.

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