Figure 7. Msh5- and Dmc1-null pachytene spermatocytes suffer from MSCI failure. (a and b) Zfy2 RNA/DNA FISH analysis showing transcription in an Msh5−/− spermatocyte with fragmented γH2AX staining, judged to be in pachytene; 59/60 (98%) transcribed Zfy2. (c and d) Ddx3x/Usp9x RNA/DNA FISH analysis showing transcription in an Msh5−/− pachytene spermatocyte with fragmented γH2AX staining; 44/49 (90%) transcribed Ddx3x/Usp9x. (e and f) Ddx3x/Usp9x RNA/DNA FISH analysis showing a nontranscribing Msh5−/− pachytene spermatocyte with the Ddx3x/Usp9x locus within a sex body–like γH2AX domain. (g and h) Zfy2 RNA/DNA FISH analysis showing transcription in a Dmc1−/− spermatocyte judged to be in pachytene; 37/39 (95%) transcribed Zfy2. (i and j) Ddx3x/Usp9x RNA/DNA FISH analysis showing two Dmc1−/− pachytene spermatocytes with fragmented γH2AX staining, one transcribing and the second nontranscribing; 37/57 (65%) transcribed Ddx3x/Usp9x. (k and l) H1t-positive Dmc1−/− pachytene spermatocyte showing Zfy2 transcription; 68/72 (92%) transcribed Zfy2. (m and n) H1t-positive Dmc1−/− pachytene spermatocyte showing Ddx3x/Usp9x transcription; 43/68 (63%) transcribed Ddx3x/Usp9x. (a–n) The white outline indicates the extent of the γH2AX domain before the DNA FISH, and arrows indicate FISH signals. Bars: (a–h and k–n) 5 μm; (i and j) 7 μm.).
Figure 7.

Msh5- and Dmc1-null pachytene spermatocytes suffer from MSCI failure. (a and b) Zfy2 RNA/DNA FISH analysis showing transcription in an Msh5−/− spermatocyte with fragmented γH2AX staining, judged to be in pachytene; 59/60 (98%) transcribed Zfy2. (c and d) Ddx3x/Usp9x RNA/DNA FISH analysis showing transcription in an Msh5−/− pachytene spermatocyte with fragmented γH2AX staining; 44/49 (90%) transcribed Ddx3x/Usp9x. (e and f) Ddx3x/Usp9x RNA/DNA FISH analysis showing a nontranscribing Msh5−/− pachytene spermatocyte with the Ddx3x/Usp9x locus within a sex body–like γH2AX domain. (g and h) Zfy2 RNA/DNA FISH analysis showing transcription in a Dmc1−/− spermatocyte judged to be in pachytene; 37/39 (95%) transcribed Zfy2. (i and j) Ddx3x/Usp9x RNA/DNA FISH analysis showing two Dmc1−/− pachytene spermatocytes with fragmented γH2AX staining, one transcribing and the second nontranscribing; 37/57 (65%) transcribed Ddx3x/Usp9x. (k and l) H1t-positive Dmc1−/− pachytene spermatocyte showing Zfy2 transcription; 68/72 (92%) transcribed Zfy2. (m and n) H1t-positive Dmc1−/− pachytene spermatocyte showing Ddx3x/Usp9x transcription; 43/68 (63%) transcribed Ddx3x/Usp9x. (a–n) The white outline indicates the extent of the γH2AX domain before the DNA FISH, and arrows indicate FISH signals. Bars: (a–h and k–n) 5 μm; (i and j) 7 μm.).

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal