Figure 6. Bnip-2 and Cdo regulate Cdc42 activity. (A) GTP-bound Cdc42 (Cdc42*) in C2C12 cells proliferating in GM (G) or cultured for 48 h in DM (D) was pulled down from cell lysates with GST-PAK-1 PBD beads and Western blotted with Cdc42 antibodies (top). Total levels of Cdc42 were determined by blotting straight lysates with Cdc42 antibodies (bottom). (B) Levels of GTP-bound and total Cdc42 in C2C12/Bnip-2 (+) or vector control (−) cells in GM or DM were assessed as described in A. (C) Levels of GTP-bound and total Cdc42 in C2C12 cells that express Bnip-2 siRNA (+) or an irrelevant sequence (−) in GM or DM were assessed as described in A. (D) Lysates of C2C12 cells cultured in GM to ∼80% confluence and then transferred to DM for the indicated times were immunoprecipitated with Cdo antibodies and then Western blotted with Cdc42 or Cdo antibodies. (E) Levels of GTP-bound and total Cdc42 in C2C12 cells that express Cdo siRNA (+) or an irrelevant sequence (−) in GM or DM were assessed as described in A. The arrowhead indicates a nonspecific band. (F) Levels of GTP-bound and total Cdc42 in myoblasts of the indicated Cdo genotype cultured in GM or DM assessed as described in A.
Figure 6.

Bnip-2 and Cdo regulate Cdc42 activity. (A) GTP-bound Cdc42 (Cdc42*) in C2C12 cells proliferating in GM (G) or cultured for 48 h in DM (D) was pulled down from cell lysates with GST-PAK-1 PBD beads and Western blotted with Cdc42 antibodies (top). Total levels of Cdc42 were determined by blotting straight lysates with Cdc42 antibodies (bottom). (B) Levels of GTP-bound and total Cdc42 in C2C12/Bnip-2 (+) or vector control (−) cells in GM or DM were assessed as described in A. (C) Levels of GTP-bound and total Cdc42 in C2C12 cells that express Bnip-2 siRNA (+) or an irrelevant sequence (−) in GM or DM were assessed as described in A. (D) Lysates of C2C12 cells cultured in GM to ∼80% confluence and then transferred to DM for the indicated times were immunoprecipitated with Cdo antibodies and then Western blotted with Cdc42 or Cdo antibodies. (E) Levels of GTP-bound and total Cdc42 in C2C12 cells that express Cdo siRNA (+) or an irrelevant sequence (−) in GM or DM were assessed as described in A. The arrowhead indicates a nonspecific band. (F) Levels of GTP-bound and total Cdc42 in myoblasts of the indicated Cdo genotype cultured in GM or DM assessed as described in A.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal