Figure 5. Bnip-2 deletion mutants defective in binding Cdc42 and Cdo do not promote myogenic differentiation. (A) C2C12 cells were cotransfected with control, Bnip-2 or Bnip-2 deletion mutant expression vectors, and pQ-lacZ (a vector that drives expression of nuclear-localized β-gal to mark transfectants). Differentiated cultures were double stained for β-gal activity (blue) and for MHC expression (brown). Bar, 0.1 mm. (B) Quantification of C2C12 cell differentiation shown in A. Cultures were scored as MHC− or MHC+, with MHC+ cells further scored as having a single (1) nucleus, two to four nuclei, or greater than or equal to five nuclei. Values represent means of triplicate determinations ±1 SD. The experiment was repeated three times with similar results.
Figure 5.

Bnip-2 deletion mutants defective in binding Cdc42 and Cdo do not promote myogenic differentiation. (A) C2C12 cells were cotransfected with control, Bnip-2 or Bnip-2 deletion mutant expression vectors, and pQ-lacZ (a vector that drives expression of nuclear-localized β-gal to mark transfectants). Differentiated cultures were double stained for β-gal activity (blue) and for MHC expression (brown). Bar, 0.1 mm. (B) Quantification of C2C12 cell differentiation shown in A. Cultures were scored as MHC− or MHC+, with MHC+ cells further scored as having a single (1) nucleus, two to four nuclei, or greater than or equal to five nuclei. Values represent means of triplicate determinations ±1 SD. The experiment was repeated three times with similar results.

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