Figure 1. Cdo interacts with Bnip-2. (A) Yeast transformed with the indicated vectors for Gal4 DNA binding domain (BD) fused to the transmembrane (TM) plus intracellular regions (ICR) of Cdo or Necl-2 and Gal4 activation domain (AD) fused to Bnip-2 or Pals2 were plated on two-hybrid interaction-dependent selective medium. (B) Lysates of 293T cells transiently transfected with flag-tagged Bnip-2, Cdo, or control (−) expression vectors as indicated were immunoprecipitated (IP) with flag antibodies and then Western blotted with flag or Cdo antibodies. (C) Lysates of C2C12 cells cultured in GM to ∼80% confluence and then transferred to DM for the indicated times were subjected to Western blot analysis with the indicated antibodies. (D) Lysates of C2C12 cells that were proliferating in GM (G) or transferred to DM for the indicated times were immunoprecipitated with Bnip-2 antibodies and then Western blotted with Cdo or Bnip-2 antibodies.
Figure 1.

Cdo interacts with Bnip-2. (A) Yeast transformed with the indicated vectors for Gal4 DNA binding domain (BD) fused to the transmembrane (TM) plus intracellular regions (ICR) of Cdo or Necl-2 and Gal4 activation domain (AD) fused to Bnip-2 or Pals2 were plated on two-hybrid interaction-dependent selective medium. (B) Lysates of 293T cells transiently transfected with flag-tagged Bnip-2, Cdo, or control (−) expression vectors as indicated were immunoprecipitated (IP) with flag antibodies and then Western blotted with flag or Cdo antibodies. (C) Lysates of C2C12 cells cultured in GM to ∼80% confluence and then transferred to DM for the indicated times were subjected to Western blot analysis with the indicated antibodies. (D) Lysates of C2C12 cells that were proliferating in GM (G) or transferred to DM for the indicated times were immunoprecipitated with Bnip-2 antibodies and then Western blotted with Cdo or Bnip-2 antibodies.

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