Figure 3. ATPase, DEAH, or helicase motifs of Mph1p are dispensable for GCR-promoting activity and synergistic sensitivity to MMS with the srs2Δ mutation. (A) Locations of mutations used in this study. (B) The overexpression of ATPase, DEAH, or helicase mutant Mph1p proteins still showed strong GCR enhancement similar to what was achieved by the overexpression of wild-type Mph1p. The GCR rates are provided in Table S2 (available). (C) γ ray and MMS sensitivities caused by excess Mph1p remain when mutant Mph1p proteins were overexpressed. (D) The synergistic MMS sensitivity by the mph1Δ mutation in the srs2Δ strain was rescued by the Mph1ps carrying a mutation in the helicase or DEAH motifs. Rates are presented as the mean of two median values with standard deviation.
Figure 3.

ATPase, DEAH, or helicase motifs of Mph1p are dispensable for GCR-promoting activity and synergistic sensitivity to MMS with the srs2Δ mutation. (A) Locations of mutations used in this study. (B) The overexpression of ATPase, DEAH, or helicase mutant Mph1p proteins still showed strong GCR enhancement similar to what was achieved by the overexpression of wild-type Mph1p. The GCR rates are provided in Table S2 . (C) γ ray and MMS sensitivities caused by excess Mph1p remain when mutant Mph1p proteins were overexpressed. (D) The synergistic MMS sensitivity by the mph1Δ mutation in the srs2Δ strain was rescued by the Mph1ps carrying a mutation in the helicase or DEAH motifs. Rates are presented as the mean of two median values with standard deviation.

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