Figure 8. JNK1/cJun activation plays a crucial role in collagen I–mediated changes in BxPC3 cells. (A) Mock BxPC3 cells or cells expressing MKK7-JNK1 (constitutively active JNK1) or MKK7-KM-JNK1 (inactive control) were extracted 2 d after plating on noncoated or collagen I–coated dishes. 30 or 60 μg of protein was blotted for N-cadherin, HA tag (to detect MKK7/JNK1), or tubulin. (B) BxPC3 cells expressing MKK7-JNK1 (a and b) or MKK7-KM-JNK1 (c and d) were cultured on noncoated (a and c) or collagen I–coated (b and d) dishes for 2 d. Bar, 100 μm. (C) 30 μg of protein from mock BxPC3 cells or cells expressing dominant-negative cJun (cJun-DN) were extracted 2 d after plating on noncoated or collagen I–coated dishes, resolved by SDS-PAGE, and blotted for N-cadherin, total cJun, or tubulin. (D) Mock BxPC3 cells (a and b) or cells expressing cJun-DN (c and d) were cultured on noncoated (a and c) or collagen I–coated (b and d) dishes for 2 d. Bar, 100 μm.
Figure 8.

JNK1/cJun activation plays a crucial role in collagen I–mediated changes in BxPC3 cells. (A) Mock BxPC3 cells or cells expressing MKK7-JNK1 (constitutively active JNK1) or MKK7-KM-JNK1 (inactive control) were extracted 2 d after plating on noncoated or collagen I–coated dishes. 30 or 60 μg of protein was blotted for N-cadherin, HA tag (to detect MKK7/JNK1), or tubulin. (B) BxPC3 cells expressing MKK7-JNK1 (a and b) or MKK7-KM-JNK1 (c and d) were cultured on noncoated (a and c) or collagen I–coated (b and d) dishes for 2 d. Bar, 100 μm. (C) 30 μg of protein from mock BxPC3 cells or cells expressing dominant-negative cJun (cJun-DN) were extracted 2 d after plating on noncoated or collagen I–coated dishes, resolved by SDS-PAGE, and blotted for N-cadherin, total cJun, or tubulin. (D) Mock BxPC3 cells (a and b) or cells expressing cJun-DN (c and d) were cultured on noncoated (a and c) or collagen I–coated (b and d) dishes for 2 d. Bar, 100 μm.

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