MLK3 is necessary for collagen I–induced changes in BxPC3 cells. (A) 30 μg of protein from BxPC3 cells expressing shEGFP or shMLK3 extracted 2 d after plating on noncoated or collagen I–coated dishes was resolved by SDS-PAGE and blotted for total MLK3, N-cadherin, or tubulin. (B) BxPC3 cells expressing shEGFP (a and b) or shMLK3 (c and d) were cultured on noncoated (a and c) or collagen I–coated (b and d) dishes for 2 d. Bar, 100 μm. (C) In vitro kinase assays were performed using cells expressing shEGFP or shMLK3. Kinase activity was detected using p-c-Jun pAb (S73).