p130CAS plays a key role in collagen I–induced changes in BxPC3 cells. (A) 600 μg of protein from BxPC3 cells 12 h after plating on noncoated or collagen I–coated dishes was immunoprecipitated with rabbit IgG or anti-DDR1 rabbit pAb and blotted for p130CAS or DDR1. (B) 600 μg of protein from BxPC3 cells 12 h after plating on noncoated or collagen I–coated dishes was immunoprecipitated with mouse IgG or anti-p130CAS mouse mAb and blotted for DDR1 or p130CAS. (C) BxPC3 cells expressing the neomycin resistance gene (mock), Src*/CAS(SD) (dominant-negative p130CAS), or SrcKM/CAS(SD) (inactive control for dominant-negative p130CAS) were extracted in RIPA buffer 2 d after plating on noncoated or collagen I–coated dishes. 30 μg of protein was resolved by SDS-PAGE and blotted for N-cadherin, myc tag (to detect p130CAS), or tubulin. (D) Mock BxPC3 cells (a and b), cells expressing Src*/CAS(SD) (c and d), or cells expressing SrcKM/CAS(SD) (e and f) were cultured on noncoated (a, c, and e) or collagen I–coated dishes (b, d, and f) for 2 d. Bar, 100 μm.