Figure 2. Inhibition of integrin signaling in BxPC3 cells. (A) RIPA extracts (30 μg of protein) from cells expressing shEGFP (control) or shIntegrin β1 (shIntβ1) cultured on noncoated or collagen I–coated dishes for 2 d were resolved by SDS-PAGE and blotted for integrin β1 (a), N-cadherin (b), or tubulin (f). 60 μg of protein from cells cultured on noncoated or collagen I–coated dishes for 4 h was blotted for phospho-FAK (Y577; c), total FAK (d), or phospho-Pyk2 (Y579/Y580; e). (B) BxPC3 cells expressing shEGFP (a and b), shIntegrin β1 (c and d), FRNK (e and f), both FRNK and shPyk2 (g and h), or shPyk2 alone (i and j) were cultured on noncoated (a, c, e, and g) or collagen I–coated (b, d, f, and h–j) dishes for 2 d. Bar, 100 μm. (C) RIPA extracts from cells expressing shEGFP or both shPyk2 and FRNK were blotted for phospho-FAK (Y577; a), total FAK (b), phospho-Pyk2, (Y579/Y580; c), total Pyk2 (d), N-cadherin (e), or tubulin (f).
Figure 2.

Inhibition of integrin signaling in BxPC3 cells. (A) RIPA extracts (30 μg of protein) from cells expressing shEGFP (control) or shIntegrin β1 (shIntβ1) cultured on noncoated or collagen I–coated dishes for 2 d were resolved by SDS-PAGE and blotted for integrin β1 (a), N-cadherin (b), or tubulin (f). 60 μg of protein from cells cultured on noncoated or collagen I–coated dishes for 4 h was blotted for phospho-FAK (Y577; c), total FAK (d), or phospho-Pyk2 (Y579/Y580; e). (B) BxPC3 cells expressing shEGFP (a and b), shIntegrin β1 (c and d), FRNK (e and f), both FRNK and shPyk2 (g and h), or shPyk2 alone (i and j) were cultured on noncoated (a, c, e, and g) or collagen I–coated (b, d, f, and h–j) dishes for 2 d. Bar, 100 μm. (C) RIPA extracts from cells expressing shEGFP or both shPyk2 and FRNK were blotted for phospho-FAK (Y577; a), total FAK (b), phospho-Pyk2, (Y579/Y580; c), total Pyk2 (d), N-cadherin (e), or tubulin (f).

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