Overexpression of integrin α5 in BxPC3 cells. (A) BxPC3 cells (a–c) or integrin α5–overexpressing BxPC3 cells (d–f) were cultured on noncoated (a and d), collagen I–coated (b and e), or fibronectin-coated (c and d) dishes. Bar, 100 μm. (B) 30 μg of protein from mock or integrin α5–overexpressing BxPC3 cells (2 d after seeding) was resolved by SDS-PAGE and blotted for integrin α5 (Int α5) or tubulin. (C) 60 μg of protein from mock or integrin α5–overexpressing BxPC3 cells (4 h after seeding) was resolved by SDS-PAGE and blotted for phospho-FAK (p-FAK; Y577), total FAK (t-FAK), or tubulin.