Figure 7. Dynamic interactions between rhTRIM5α cytoplasmic bodies and HIV-1 virions. (A and C) HeLa cells expressing YFP-rhTRIM5α (green) were infected with mCherry-Vpr–labeled HIV-1 virions (red). Images were acquired every 10 s for 15 min. Virus could induce the formation of new cytoplasmic bodies by becoming coated with YFP-rhTRIM5α protein derived from a preexisting cytoplasmic body (A) or by the de novo formation of a cytoplasmic body around a virion (C). (B and D) Quantifying these interactions confirms that the YFP-rhTRIM5α signal (vTRIM) becoming associated with an mCherry-Vpr–labeled virion (virus 1) in C derives from a preexisting rhTRIM5α body (TRIMbody) at the time point indicated by the arrows. The provided images were derived from a volume compression of a z stack of 10 images taken at a 0.5-μm step size at each time point.
Figure 7.

Dynamic interactions between rhTRIM5α cytoplasmic bodies and HIV-1 virions. (A and C) HeLa cells expressing YFP-rhTRIM5α (green) were infected with mCherry-Vpr–labeled HIV-1 virions (red). Images were acquired every 10 s for 15 min. Virus could induce the formation of new cytoplasmic bodies by becoming coated with YFP-rhTRIM5α protein derived from a preexisting cytoplasmic body (A) or by the de novo formation of a cytoplasmic body around a virion (C). (B and D) Quantifying these interactions confirms that the YFP-rhTRIM5α signal (vTRIM) becoming associated with an mCherry-Vpr–labeled virion (virus 1) in C derives from a preexisting rhTRIM5α body (TRIMbody) at the time point indicated by the arrows. The provided images were derived from a volume compression of a z stack of 10 images taken at a 0.5-μm step size at each time point.

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