Stable interaction between rhTRIM5α cytoplasmic bodies and HIV-1 virions. HeLa cells expressing YFP-rhTRIM5α (green) were infected with mCherry-Vpr–labeled HIV-1 virions (red). Images were acquired every 15 s for 10 min. Arrows indicate one YFP-rhTRIM5α cytoplasmic body associating and trafficking with one mCherry-Vpr–labeled virus over time. The bottom right panel shows the path traveled by the YFP-rhTRIM5α– and mCherry-Vpr–labeled virion throughout the experiment. The provided image is one z section from a deconvolved z-stack image showing the z section in which the interaction is currently trafficking.