HIV-1 interacts with rhTRIM5α cytoplasmic bodies in the absence of drug. (A) rhTRIM5α-HA–expressing HeLa cells were continuously infected with GFP-Vpr–labeled HIV-1 virions (green) for 2 h. Cells were fixed and immunostained with anti-HA antibody (red) or Hoechst to visualize cellular DNA (blue). Arrows indicate HIV-1 virions associated with rhTRIM5α cytoplasmic bodies. The boxed area in the top left panel has been enlarged in the other three panels, and the fluorescent signals for either rhTRIM5α, GFP-Vpr HIV-1, or both (merge) are shown for clarity. The provided image is one z section from a deconvolved z-stack image.