Akt localizes to the embryonic cortex in a cell cycle–dependent manner. (A) Western blot of 0–3-h wild-type embryos and embryos containing UAS-Akt-turbo-GFP driven by Nanos Gal4. Akt-tGFP is expressed at similar levels to endogenous Akt. Molecular mass (kD) is indicated on the left. (B) Live confocal analysis of embryos expressing Akt-tGFP under the UAS-Gal4 system. Akt-tGFP appears to cycle between cortical caps (0 and 1,770 s) and pseudocleavage furrows (710 and 930 s). Time is indicated in seconds. See Video 1 . Bar, 10 μm.