Cellular composition of sciatic nerves in Sox10^ΔeSC mice. (A–L) Immunohistochemistry was performed on sciatic nerve sections from wild-type (wt; A, C, E, G, I, and K) and Sox10^ΔeSC (B, D, F, H, J, and L) mice at P16 using antibodies directed against Pecam (A–D) and von Willebrand factor (vWF; C and D) as predominantly endothelial markers, desmin (E–H) as a marker for pericytes, Iba1 (I and J) as a marker for macrophages, and CD3 (K and L) as a marker for T lymphocytes and is shown in low (A, B, E, and F) and high (C, D, and G–L) magnification. All markers are in red except Pecam, to which green color was assigned. Nuclei in C, D, and G–L were counterstained by DAPI (blue). (M–O) These and analogous experiments at other time points were used to determine the cellular composition of sciatic nerves in wild-type and Sox10^ΔeSC mice at P16 (M), P24 (N), and P32 (O). Quantifications were performed on at least 20 sections from three mice per genotype. Cell numbers are presented as mean ± SEM. Bars: (A, B, E, and F) 50 µm; (C, D, and G–L) 10 µm.