Figure 6.
Figure 6. V100 interacts with the endosomal Syx7/Avl but not the Golgi/lysosomal Syx16. (A) Immunoblots of co-IP with anti-V100 antibody from adult fly head extract, probed with antibodies against Syx1A, Syx7/Avl, and Syx16. IgG, analogous co-IPs using preimmunoserum instead of anti-V100 serum. (B) Co-IP with anti-Syx7/Avl antibody from adult fly head extract probed with anti-V100. (C) Pull-down from adult fly head extract using bacterially made His-V100–N terminus. Immunoblot probed with anti-Syx1A, -Syx7, and -Syx16. (D) Pull-down from adult fly head extract using bacterially made His-Syx7. As negative control (His-CtrlProt), we used a bacterially made His fusion to a fly lipid enzyme (CG8630). Immunoblot probed with anti-V100. (E) GST pull-down of bacterially made His-Syx7 with bacterially made GST-V100–N terminus.

V100 interacts with the endosomal Syx7/Avl but not the Golgi/lysosomal Syx16. (A) Immunoblots of co-IP with anti-V100 antibody from adult fly head extract, probed with antibodies against Syx1A, Syx7/Avl, and Syx16. IgG, analogous co-IPs using preimmunoserum instead of anti-V100 serum. (B) Co-IP with anti-Syx7/Avl antibody from adult fly head extract probed with anti-V100. (C) Pull-down from adult fly head extract using bacterially made His-V100–N terminus. Immunoblot probed with anti-Syx1A, -Syx7, and -Syx16. (D) Pull-down from adult fly head extract using bacterially made His-Syx7. As negative control (His-CtrlProt), we used a bacterially made His fusion to a fly lipid enzyme (CG8630). Immunoblot probed with anti-V100. (E) GST pull-down of bacterially made His-Syx7 with bacterially made GST-V100–N terminus.

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