Figure 5. Cortactin is required for parkin-CCCP–induced mitochondrial clearance. MEFs were transfected with control or cortactin RNAi and parkin-GFP, and incubated with or without CCCP for 16 h as indicated. (A) Cells were subjected to immunostaining with antibodies for Tom20 and parkin. Bar, 10 µm. (B) Cell lysates were subjected to immunoblotting with antibodies for Tom20, parkin, cortactin, and actin.
Figure 5.

Cortactin is required for parkin-CCCP–induced mitochondrial clearance. MEFs were transfected with control or cortactin RNAi and parkin-GFP, and incubated with or without CCCP for 16 h as indicated. (A) Cells were subjected to immunostaining with antibodies for Tom20 and parkin. Bar, 10 µm. (B) Cell lysates were subjected to immunoblotting with antibodies for Tom20, parkin, cortactin, and actin.

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