Figure 6.
pbl embryos have defects in vesicle transport. Markers are indicated on the left. Genotypes are indicated on the right. All panels show live images. (A–F) Actin and Syt-GFP vesicles strongly localize to furrows during cytokinesis in WT (A and D). pbl5 embryos fail to localize Syt-GFP vesicles to the cell equator in cells with severe furrowing defects (B) and cells with weak furrowing defects (C). pbl5 embryos show weak actin recruitment to the cell equator (E and F). The furthest extent of furrow elongation is shown in B′, C′, E′, and F′. For full videos, see Videos 5, 6, and 7. Bars, 5 μm.