Live imaging shows that actin puncta associate with vesicles and endosomes. Rhodamine-actin (red) was injected into embryos expressing a distinct GFP fusion protein (green; specified in left column). Puncta was scored as colocalized if two puncta moved in unison over a minimum of two consecutive time points (arrowheads). Time points (s) are indicated at the top right of panel. Percentage of colocalization is shown in the right column. (A–F) Live series shows that actin puncta colocalize with Rab7-GFP–labeled late endosomes (A), Dah-GFP–labeled vesicles (B), Syt-GFP–labeled vesicles (C), and Rab5-GFP–labeled early endosomes (D). Actin puncta did not show considerable colocalization with Grasp65-GFP–labeled Golgi (E) or Clathrin light chain–GFP–labeled membrane (F). Insets in C depict single channels for actin (left) and Syt-GFP (right). Error = SEM. Bar, 5 μm