Actin puncta are targeted to ingressing furrows. (A) Phalloidin in fixed embryos shows F-actin puncta in cycle 14 interphase cells (arrow). (B) F-actin puncta localize to the cell equator near furrow MTs (arrowheads) and the central spindle at cytokinesis (arrows). Merged images of F-actin (phalloidin; red) and MTs (α-Tub; green) are shown in the bottom panels. (C–J) Live series of cycle 14 telophase. Rhodamine-actin, red; Tub-GFP, green. Actin puncta accumulate near the central spindle (D, bracket). Arrowheads in C–F show an individual actin puncta moving alongside an MT bundle at the central spindle. Furrow MTs (G, arrowed bracket) align perpendicular to the cleavage plane. Its midpoint contacts the furrow tip (G, arrowhead). Actin puncta are visible near ends of furrow MTs (I, blue arrowhead) and accumulate at the contact point between the leading furrow edge and furrow MTs (G and I, white arrowheads). Arrow in I shows an actin puncta colocalized with the central spindle. Time points (s) are shown at the top right of each panel. For full video, see Video 2. Bars, 5 μm.