Vesicles and membrane are targeted to furrows during cytokinesis in cycle 14 cells. All images are from living embryos. (A) Syt-GFP–labeled vesicles. Arrows indicate a single vesicle. Arrowheads indicate other vesicles moving toward the furrow. Time points (s) are shown at the top left of each panel. For the full video, see Video 1. (B) n-Syb–GFP–labeled vesicles show strong furrow enrichment at cytokinesis (arrowhead). (C) Dah-GFP vesicles show cytoplasmic puncta at the cell equator and furrow enrichment at cytokinesis (arrows). (D) The general membrane dye FM1-43 accumulates at the cell equator during cytokinesis. Arrows, cleavage plane. (E) Grasp-65-GFP–labeled Golgi do not accumulate at the cell equator at cytokinesis. Arrows, cleavage plane. Outline, cell cortex. (F) RhoA-GFP. (G) Percentage of vesicles localized to polar regions of the cell (outer) and to the middle of the cell (center). Cells were divided into thirds (inset). Vesicle localization was scored at cell stages indicated on the x axis. n = 92 (late metaphase), 74 (anaphase B), 96 (furrow initiation), 91 (elongation), or 100 (scission). Bars, 5 μm.