Figure 5. Defective DNA repair in hCdc14AΔ/Δneo or hCdc14BΔ/Δ cells. (A and B) The indicated cell lines were fixed and stained for pH3 (red) and γ-H2A.X (green) and examined by microscopy. Mitotic cells are shown. Bars, 10 µm. (C and D) Quantification of the γ-H2A.X staining in A and B (n = 3; 100 mitotic cells per genotype). (E) Quantification of γ-H2A.X foci in mitotic cells (n = 3; 20 mitotic cells per genotype) as in Fig. 2 E. (F and G) Cell viability analyzed by MTT assay 48 h after treatment with IR (n = 3). Cells lacking hCdc14A or hCdc14B are significantly more sensitive than control cells (P < 0.02 and P < 0.01, respectively). Error bars indicate mean ± SD.
Figure 5.

Defective DNA repair in hCdc14AΔ/Δneo or hCdc14BΔ/Δ cells. (A and B) The indicated cell lines were fixed and stained for pH3 (red) and γ-H2A.X (green) and examined by microscopy. Mitotic cells are shown. Bars, 10 µm. (C and D) Quantification of the γ-H2A.X staining in A and B (n = 3; 100 mitotic cells per genotype). (E) Quantification of γ-H2A.X foci in mitotic cells (n = 3; 20 mitotic cells per genotype) as in Fig. 2 E. (F and G) Cell viability analyzed by MTT assay 48 h after treatment with IR (n = 3). Cells lacking hCdc14A or hCdc14B are significantly more sensitive than control cells (P < 0.02 and P < 0.01, respectively). Error bars indicate mean ± SD.

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