Thin-section EM analysis of the NE and NPCs in wt and pom33Δ cells. wt cells (A–C) and pom33Δ cells (D–J) were grown to early log phase at 30°C and processed for thin-section EM (as described in Materials and methods). Arrowheads point to typical NPCs and asterisks denote distorted NPC-like structures. Bar, 500 nm.