Figure 6. The pom33Δ mutant displays an NPC clustering phenotype. (A) The localization of Nup84-GFP was analyzed in wt and pom33Δ cells carrying the Nup49-mCherry plasmid. Wide-field images of single-channel fluorescence for mCherry and GFP; merge and DIC images are also shown. (B) The localization of the indicated GFP-tagged Nups was analyzed by direct fluorescence microscopy in wt and pom33Δ cells. The bright Ndc1-GFP dots in wt and pom33Δ cells correspond to the spindle pole body. (C) Fluorescence microscopy analysis of the localization of the inner nuclear membrane protein Asi1-GFP in wt and pom33Δ cells expressing Nup49-mCherry. Bars, 5 µm.
Figure 6.

The pom33Δ mutant displays an NPC clustering phenotype. (A) The localization of Nup84-GFP was analyzed in wt and pom33Δ cells carrying the Nup49-mCherry plasmid. Wide-field images of single-channel fluorescence for mCherry and GFP; merge and DIC images are also shown. (B) The localization of the indicated GFP-tagged Nups was analyzed by direct fluorescence microscopy in wt and pom33Δ cells. The bright Ndc1-GFP dots in wt and pom33Δ cells correspond to the spindle pole body. (C) Fluorescence microscopy analysis of the localization of the inner nuclear membrane protein Asi1-GFP in wt and pom33Δ cells expressing Nup49-mCherry. Bars, 5 µm.

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