POM33 displays genetic interactions with core components of NPC. (A) Growth properties of wt, pom33Δ, per33Δ, nup133Δ, and of corresponding double and triple mutants. Equivalent amounts of cells were spotted as fivefold dilutions on YEPD plates, and were incubated for 5 d at 18°C or for 3 d at 25, 30, and 37°C. (B) Summary of the genetic interactions between pom33Δ, per33Δ, or pom33Δ per33Δ, and Nups or NE/ER mutants. Synthetic interactions were scored as: SS, strong synergistic interaction; S, synergistic interaction; V, viable; n.d., not determined. The temperature above which the synthetic phenotype was observed is indicated. Footnote a, see Fig. 4 A; footnote b, see Fig. S2; *, note that PER33 deletion partially rescues the pom33Δ nup133Δ synthetic phenotype at 18°C. (C) Growth assay of ndc1-39 mutant cells transformed with an empty pRS315 plasmid (ø), with centromeric (cen), or with multicopy (2µ) plasmids encompassing the POM33 or PER33 genes. Transformants were spotted as fivefold dilutions on selective medium, and plates were incubated for 3 d at the indicated temperatures.