scFv-EM48 reduces the distribution of mutant htt in the neurites of PC12 cells. (A) PC12 cells were infected by adenoviral htt-130Q only. Two images (right and left) from independent experiments are shown. The middle image is a merged image showing nuclear (blue) staining for the left image. Arrows indicate htt aggregates in neurites. (B) PC12 cells were coinfected with adenoviral htt and adenoviral scFv-EM48-HA and then stained with rabbit EM48 for htt and mouse anti-HA for scFv-EM48. (top) Cells coexpressing htt-130Q and scFv-EM48. (bottom) Cells coexpressing htt-23Q and scFv-EM48. Note that the neuritic aggregates formed by htt-130Q are reduced by scFv-EM48. Bars, 10 μm. (C) Quantitative assessment of the percentage of transfected PC12 cells showing neuritic aggregates and the ratios of neuritic htt signals to cell body signals. The data are presented as means ± standard error. **, P < 0.01.