Figure 8. SUMOylation of CASK influences dendritic spine morphology. (A) Hippocampal neurons were cotransfected with GFP-actin and Myc-CASK, Myc-C-SUMO1-CASK, or vector control at a ratio of 1:5 at 10–12 DIV as indicated and were fixed at 17–18 DIV to examine spine morphology by double staining using Myc and GFP antibodies. Only double-positive neurons were collected for analysis. The images of GFP signal are shown. Myc tag signal revealing the patterns of CASK and C-SUMO1-CASK are shown in Fig. S3 (available). The bottom panel shows higher magnification of the boxed areas in the top panel. (B) Quantification of spine density, length, and width from A. The histograms show the cumulative distribution as a percentage. A total of 2,828 (GFP-actin alone), 5,229 (Myc-CASK), and 3,586 (Myc-C-SUMO1-CASK) spines from >117 dendrites of >22 neurons were analyzed. Mean values ± SEM for each group are shown in the insets. Bars: (A, top) 20 μm; (A, bottom) 2 μm.
Figure 8.

SUMOylation of CASK influences dendritic spine morphology. (A) Hippocampal neurons were cotransfected with GFP-actin and Myc-CASK, Myc-C-SUMO1-CASK, or vector control at a ratio of 1:5 at 10–12 DIV as indicated and were fixed at 17–18 DIV to examine spine morphology by double staining using Myc and GFP antibodies. Only double-positive neurons were collected for analysis. The images of GFP signal are shown. Myc tag signal revealing the patterns of CASK and C-SUMO1-CASK are shown in Fig. S3 . The bottom panel shows higher magnification of the boxed areas in the top panel. (B) Quantification of spine density, length, and width from A. The histograms show the cumulative distribution as a percentage. A total of 2,828 (GFP-actin alone), 5,229 (Myc-CASK), and 3,586 (Myc-C-SUMO1-CASK) spines from >117 dendrites of >22 neurons were analyzed. Mean values ± SEM for each group are shown in the insets. Bars: (A, top) 20 μm; (A, bottom) 2 μm.

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