Figure 6.
CD19 and CD38 form synapses upon stimulation and migrate together to the IgM-BCR. (A–C) Fab-PLA study of the proximity of CD19 to CD38 on unstimulated (0 min; A), or 5- and 15-min α-IgM–stimulated PB B cells (B). PLA signals are shown in red and nuclei in blue. Scale bar, 5 µm. (C) Scatter dot plot represents the mean of PLA signals for CD19:CD38 interaction (signal counts). (D) Schematic drawing of the proposed localization and interaction of IgM, CD19, and CD38 in unstimulated and α-IgM–stimulated B cells without and with exposure to DARA. The experiment was repeated four times. Shown are representative microscope images. (C) Kruskal-Wallis test corrected for Dunn’s multiple comparisons was used (****, P < 0.0001). In these graphs, every data point is one cell; error bars show mean ± SD.

CD19 and CD38 form synapses upon stimulation and migrate together to the IgM-BCR. (A–C) Fab-PLA study of the proximity of CD19 to CD38 on unstimulated (0 min; A), or 5- and 15-min α-IgM–stimulated PB B cells (B). PLA signals are shown in red and nuclei in blue. Scale bar, 5 µm. (C) Scatter dot plot represents the mean of PLA signals for CD19:CD38 interaction (signal counts). (D) Schematic drawing of the proposed localization and interaction of IgM, CD19, and CD38 in unstimulated and α-IgM–stimulated B cells without and with exposure to DARA. The experiment was repeated four times. Shown are representative microscope images. (C) Kruskal-Wallis test corrected for Dunn’s multiple comparisons was used (****, P < 0.0001). In these graphs, every data point is one cell; error bars show mean ± SD.

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