Biolayer interferometry affinity measurements. (A and B) Graphs depict affinity measurements of anti-Gamma monoclonal antibodies for Wuhan-Hu-1 RBD (A) or N417/484K/501Y RBD (B); data are representative of experiments performed in triplicate. (C) Heat maps of two biolayer interferometry competition experiments showing relative inhibition of binding of monoclonal antibody (x axis) to preformed complexes of 417N/484K/501Y RBD with another monoclonal antibody (y axis). Gray indicates no binding; yellow indicates low binding; orange, intermediate binding; and red, high binding. Data are normalized by subtraction of autologous antibody control. Related to Fig. 3.