Antibody sequence hydrophobicity and CDR3 length. (A) Distribution of hydrophobicity GRAVY scores at the IGH CDR3 of antibodies from all donors combined and compared to human repertoire (Briney et al., 2019). (B) CDR3 lengths for all heavy and light chains of antibodies isolated in this study compared to human repertoire. For A and B, statistical significance is determined by two-sided binomial test with unequal variance and denoted by asterisks (*, P < 0.05; ****, P < 0.0001). Related to Fig. 2.