Antibody V gene frequency and mutations. (A–C) Bar graphs show the frequency distributions of human V genes for heavy chain (A), kappa chain (B), and lambda chain (C) in antibodies from Gamma-infected donors (blue) and Sequence Read Archive accession SRP010970 (orange). Statistical significance determined by two-sided binomial test with unequal variance; significant differences are denoted by asterisks (*, P < 0.05; **, P < 0.01; ****, P < 0.0001). (D) Number of somatic nucleotide mutations in IGVH (P = 0.0253 for BRA14 versus BRA15), IGVK and IGVL combined (P = 0.0177 for BRA13 versus BRA15), and total IGV (P = 0.0162 for BRA13 versus BRA15; P = 0.0091 for BRA14 versus BRA15) as indicated per donor. Red horizontal bars indicate mean values; statistical differences determined by two-sided Kruskal-Wallis test with subsequent Dunn’s multiple comparisons.