Identification of cross-reactive anti – Wuhan-Hu-1 and anti-417N/484K/501Y RBD antibodies from Gamma-infected individuals. (A) Gating strategy. Gating was performed on singlets that were CD20⁺ and CD3⁻ CD8⁻ CD14⁻ CD16⁻ Ova⁻. Sorted cells were Wuhan-Hu-1 RBD-PE⁺ and 417N/484K/501Y RBD-AF647⁺. (B) Representative flow cytometry plots showing PE-RBD– and AF-647-RBD–binding B cells for one control and three study donors. Gating strategy is shown in Fig. S2 A. (C) Distribution of antibody sequences obtained from three donors. The number in the inner circle indicates the number of sequences analyzed per individual. White indicates sequences isolated once, while gray slices are proportional to the number of clonally expanded sequences. The outer black arc denotes the frequency of clonal sequences per donor. Related to Fig. 2.